n terminal his6 sumo tev fusion vector Search Results


pet  (Addgene inc)
93
Addgene inc pet
Pet, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/pET+His6+Sumo+TEV+LIC+cloning+vector+(2S-T)+(Plasmid+%2329711)/pm39933617-166-14-15
Average 93 stars, based on 1 article reviews
pet - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
lbucas13a expression vector  (Addgene inc)
94
Addgene inc lbucas13a expression vector
Lbucas13a Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/pC0072+LbuCas13a+His6-TwinStrep-SUMO-BsaI+(Plasmid+%23115267)/med_rxiv__2021__08__02__21261509-99-2-22
Average 94 stars, based on 1 article reviews
lbucas13a expression vector - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
pet expression vector  (Addgene inc)
92
Addgene inc pet expression vector
Pet Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/pET+His6+TEV+expression+vector+with+BioBrick+polypromoter+restriction+sites+(14-B)+(Plasmid+%2348308)/us11300640-1432-8-16
Average 92 stars, based on 1 article reviews
pet expression vector - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
vectors pbig1a nsp12 his 6 3 xflag  (Addgene inc)
93
Addgene inc vectors pbig1a nsp12 his 6 3 xflag
( A ) Purified SARS-CoV-2 RdRp proteins expressed in baculovirus-infected insect cells ( Spodoptera frugiperda , Sf) analysed by SDS–PAGE and Coomassie staining. 7H8: nsp7-His 6 -nsp8, nsp12-HF: nsp12-His 6 <t>-3xFlag,</t> nsp12-F/7H8: nsp12-3xFlag/nsp7-His 6 -nsp8, nsp12-HF/7L8: nsp12-His 6 -3xFlag/nsp7-GGSGGS-nsp8, nsp12-HF/7/8: nsp12-His 6 -3xFlag/nsp7/nsp8. ( B ) Bacterially expressed and purified SARS-CoV-2 nsp7, nsp8 and nsp12 proteins analysed by SDS–PAGE and Coomassie staining. The proteins were expressed as 14His-SUMO fusion proteins in E. coli . 14His-SUMO was removed by a SUMO-specific protease during purification generating native N-termini. ( C ) Gel-based primer-extension assay to test RNA-dependent RNA synthesis using the RdRp preparation Sf nsp12-F/7H8. The substrate consists of a 10 nt RNA primer annealed to the 3′ end of a 35 nt RNA template. The 5′ end of the template strand is labelled with a Cy3 fluorophore. Reaction products were analysed by native PAGE and visualisation of Cy3 fluorescence. Formation of duplex RNA by RdRp was observed over time. Controls: a preformed Cy3-labelled dsRNA with the same size as the reaction product (dsRNA), the primed substrate (no protein). ( D ) Schematic diagram illustrating the FRET-based RdRp strand displacement assay. The RNA substrate is composed of a Cy3 fluorophore-containing template strand, an annealed primer and an annealed quencher strand with a 5′ flap. RdRp activity synthesises RNA by extending the primer strand and displaces the quencher strand. The displaced quencher strand can no longer anneal to fully synthesised duplex RNA leading to an increase in Cy3 fluorescent signal. ( E ) FRET-based strand displacement assay using the indicated concentrations of Sf nsp12-F/7H8.
Vectors Pbig1a Nsp12 His 6 3 Xflag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/pBIG1a+(Plasmid+%2380611)/pmc08286815-172-1-17
Average 93 stars, based on 1 article reviews
vectors pbig1a nsp12 his 6 3 xflag - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
his6-tagged pkc wt  (Baier labs)
90
Baier labs his6-tagged pkc wt
( A ) Purified SARS-CoV-2 RdRp proteins expressed in baculovirus-infected insect cells ( Spodoptera frugiperda , Sf) analysed by SDS–PAGE and Coomassie staining. 7H8: nsp7-His 6 -nsp8, nsp12-HF: nsp12-His 6 <t>-3xFlag,</t> nsp12-F/7H8: nsp12-3xFlag/nsp7-His 6 -nsp8, nsp12-HF/7L8: nsp12-His 6 -3xFlag/nsp7-GGSGGS-nsp8, nsp12-HF/7/8: nsp12-His 6 -3xFlag/nsp7/nsp8. ( B ) Bacterially expressed and purified SARS-CoV-2 nsp7, nsp8 and nsp12 proteins analysed by SDS–PAGE and Coomassie staining. The proteins were expressed as 14His-SUMO fusion proteins in E. coli . 14His-SUMO was removed by a SUMO-specific protease during purification generating native N-termini. ( C ) Gel-based primer-extension assay to test RNA-dependent RNA synthesis using the RdRp preparation Sf nsp12-F/7H8. The substrate consists of a 10 nt RNA primer annealed to the 3′ end of a 35 nt RNA template. The 5′ end of the template strand is labelled with a Cy3 fluorophore. Reaction products were analysed by native PAGE and visualisation of Cy3 fluorescence. Formation of duplex RNA by RdRp was observed over time. Controls: a preformed Cy3-labelled dsRNA with the same size as the reaction product (dsRNA), the primed substrate (no protein). ( D ) Schematic diagram illustrating the FRET-based RdRp strand displacement assay. The RNA substrate is composed of a Cy3 fluorophore-containing template strand, an annealed primer and an annealed quencher strand with a 5′ flap. RdRp activity synthesises RNA by extending the primer strand and displaces the quencher strand. The displaced quencher strand can no longer anneal to fully synthesised duplex RNA leading to an increase in Cy3 fluorescent signal. ( E ) FRET-based strand displacement assay using the indicated concentrations of Sf nsp12-F/7H8.
His6 Tagged Pkc Wt, supplied by Baier labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/his6+tagged+pkc+wt+or+mutant++a25e++constitutively+active+mutant++cf++catalytic+fragment+/pmc03556779__mmc1-79-15-32
Average 90 stars, based on 1 article reviews
his6-tagged pkc wt - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
pet his6 gst tev lic cloning vector 2g t  (Addgene inc)
93
Addgene inc pet his6 gst tev lic cloning vector 2g t
( A ) Purified SARS-CoV-2 RdRp proteins expressed in baculovirus-infected insect cells ( Spodoptera frugiperda , Sf) analysed by SDS–PAGE and Coomassie staining. 7H8: nsp7-His 6 -nsp8, nsp12-HF: nsp12-His 6 <t>-3xFlag,</t> nsp12-F/7H8: nsp12-3xFlag/nsp7-His 6 -nsp8, nsp12-HF/7L8: nsp12-His 6 -3xFlag/nsp7-GGSGGS-nsp8, nsp12-HF/7/8: nsp12-His 6 -3xFlag/nsp7/nsp8. ( B ) Bacterially expressed and purified SARS-CoV-2 nsp7, nsp8 and nsp12 proteins analysed by SDS–PAGE and Coomassie staining. The proteins were expressed as 14His-SUMO fusion proteins in E. coli . 14His-SUMO was removed by a SUMO-specific protease during purification generating native N-termini. ( C ) Gel-based primer-extension assay to test RNA-dependent RNA synthesis using the RdRp preparation Sf nsp12-F/7H8. The substrate consists of a 10 nt RNA primer annealed to the 3′ end of a 35 nt RNA template. The 5′ end of the template strand is labelled with a Cy3 fluorophore. Reaction products were analysed by native PAGE and visualisation of Cy3 fluorescence. Formation of duplex RNA by RdRp was observed over time. Controls: a preformed Cy3-labelled dsRNA with the same size as the reaction product (dsRNA), the primed substrate (no protein). ( D ) Schematic diagram illustrating the FRET-based RdRp strand displacement assay. The RNA substrate is composed of a Cy3 fluorophore-containing template strand, an annealed primer and an annealed quencher strand with a 5′ flap. RdRp activity synthesises RNA by extending the primer strand and displaces the quencher strand. The displaced quencher strand can no longer anneal to fully synthesised duplex RNA leading to an increase in Cy3 fluorescent signal. ( E ) FRET-based strand displacement assay using the indicated concentrations of Sf nsp12-F/7H8.
Pet His6 Gst Tev Lic Cloning Vector 2g T, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/pET+His6+GST+TEV+LIC+cloning+vector+(2G-T)+(Plasmid+%2329707)/bio_rxiv__2021__01__11__425814-207-11-24
Average 93 stars, based on 1 article reviews
pet his6 gst tev lic cloning vector 2g t - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
sspi digested pet his6 sumo tev lic  (Addgene inc)
93
Addgene inc sspi digested pet his6 sumo tev lic
( A ) Purified SARS-CoV-2 RdRp proteins expressed in baculovirus-infected insect cells ( Spodoptera frugiperda , Sf) analysed by SDS–PAGE and Coomassie staining. 7H8: nsp7-His 6 -nsp8, nsp12-HF: nsp12-His 6 <t>-3xFlag,</t> nsp12-F/7H8: nsp12-3xFlag/nsp7-His 6 -nsp8, nsp12-HF/7L8: nsp12-His 6 -3xFlag/nsp7-GGSGGS-nsp8, nsp12-HF/7/8: nsp12-His 6 -3xFlag/nsp7/nsp8. ( B ) Bacterially expressed and purified SARS-CoV-2 nsp7, nsp8 and nsp12 proteins analysed by SDS–PAGE and Coomassie staining. The proteins were expressed as 14His-SUMO fusion proteins in E. coli . 14His-SUMO was removed by a SUMO-specific protease during purification generating native N-termini. ( C ) Gel-based primer-extension assay to test RNA-dependent RNA synthesis using the RdRp preparation Sf nsp12-F/7H8. The substrate consists of a 10 nt RNA primer annealed to the 3′ end of a 35 nt RNA template. The 5′ end of the template strand is labelled with a Cy3 fluorophore. Reaction products were analysed by native PAGE and visualisation of Cy3 fluorescence. Formation of duplex RNA by RdRp was observed over time. Controls: a preformed Cy3-labelled dsRNA with the same size as the reaction product (dsRNA), the primed substrate (no protein). ( D ) Schematic diagram illustrating the FRET-based RdRp strand displacement assay. The RNA substrate is composed of a Cy3 fluorophore-containing template strand, an annealed primer and an annealed quencher strand with a 5′ flap. RdRp activity synthesises RNA by extending the primer strand and displaces the quencher strand. The displaced quencher strand can no longer anneal to fully synthesised duplex RNA leading to an increase in Cy3 fluorescent signal. ( E ) FRET-based strand displacement assay using the indicated concentrations of Sf nsp12-F/7H8.
Sspi Digested Pet His6 Sumo Tev Lic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/pET+His6+Sumo+TEV+LIC+cloning+vector+(1S)+(Plasmid+%2329659)/pmc12591035-252-8-11
Average 93 stars, based on 1 article reviews
sspi digested pet his6 sumo tev lic - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
pet his6 mbp tev lic cloning vector  (Addgene inc)
94
Addgene inc pet his6 mbp tev lic cloning vector
( A ) Purified SARS-CoV-2 RdRp proteins expressed in baculovirus-infected insect cells ( Spodoptera frugiperda , Sf) analysed by SDS–PAGE and Coomassie staining. 7H8: nsp7-His 6 -nsp8, nsp12-HF: nsp12-His 6 <t>-3xFlag,</t> nsp12-F/7H8: nsp12-3xFlag/nsp7-His 6 -nsp8, nsp12-HF/7L8: nsp12-His 6 -3xFlag/nsp7-GGSGGS-nsp8, nsp12-HF/7/8: nsp12-His 6 -3xFlag/nsp7/nsp8. ( B ) Bacterially expressed and purified SARS-CoV-2 nsp7, nsp8 and nsp12 proteins analysed by SDS–PAGE and Coomassie staining. The proteins were expressed as 14His-SUMO fusion proteins in E. coli . 14His-SUMO was removed by a SUMO-specific protease during purification generating native N-termini. ( C ) Gel-based primer-extension assay to test RNA-dependent RNA synthesis using the RdRp preparation Sf nsp12-F/7H8. The substrate consists of a 10 nt RNA primer annealed to the 3′ end of a 35 nt RNA template. The 5′ end of the template strand is labelled with a Cy3 fluorophore. Reaction products were analysed by native PAGE and visualisation of Cy3 fluorescence. Formation of duplex RNA by RdRp was observed over time. Controls: a preformed Cy3-labelled dsRNA with the same size as the reaction product (dsRNA), the primed substrate (no protein). ( D ) Schematic diagram illustrating the FRET-based RdRp strand displacement assay. The RNA substrate is composed of a Cy3 fluorophore-containing template strand, an annealed primer and an annealed quencher strand with a 5′ flap. RdRp activity synthesises RNA by extending the primer strand and displaces the quencher strand. The displaced quencher strand can no longer anneal to fully synthesised duplex RNA leading to an increase in Cy3 fluorescent signal. ( E ) FRET-based strand displacement assay using the indicated concentrations of Sf nsp12-F/7H8.
Pet His6 Mbp Tev Lic Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/pET+His6+MBP+TEV+LIC+cloning+vector+(1M)+(Plasmid+%2329656)/pmc08926329-263-10-24
Average 94 stars, based on 1 article reviews
pet his6 mbp tev lic cloning vector - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
438 c vector  (Addgene inc)
93
Addgene inc 438 c vector
( A ) Purified SARS-CoV-2 RdRp proteins expressed in baculovirus-infected insect cells ( Spodoptera frugiperda , Sf) analysed by SDS–PAGE and Coomassie staining. 7H8: nsp7-His 6 -nsp8, nsp12-HF: nsp12-His 6 <t>-3xFlag,</t> nsp12-F/7H8: nsp12-3xFlag/nsp7-His 6 -nsp8, nsp12-HF/7L8: nsp12-His 6 -3xFlag/nsp7-GGSGGS-nsp8, nsp12-HF/7/8: nsp12-His 6 -3xFlag/nsp7/nsp8. ( B ) Bacterially expressed and purified SARS-CoV-2 nsp7, nsp8 and nsp12 proteins analysed by SDS–PAGE and Coomassie staining. The proteins were expressed as 14His-SUMO fusion proteins in E. coli . 14His-SUMO was removed by a SUMO-specific protease during purification generating native N-termini. ( C ) Gel-based primer-extension assay to test RNA-dependent RNA synthesis using the RdRp preparation Sf nsp12-F/7H8. The substrate consists of a 10 nt RNA primer annealed to the 3′ end of a 35 nt RNA template. The 5′ end of the template strand is labelled with a Cy3 fluorophore. Reaction products were analysed by native PAGE and visualisation of Cy3 fluorescence. Formation of duplex RNA by RdRp was observed over time. Controls: a preformed Cy3-labelled dsRNA with the same size as the reaction product (dsRNA), the primed substrate (no protein). ( D ) Schematic diagram illustrating the FRET-based RdRp strand displacement assay. The RNA substrate is composed of a Cy3 fluorophore-containing template strand, an annealed primer and an annealed quencher strand with a 5′ flap. RdRp activity synthesises RNA by extending the primer strand and displaces the quencher strand. The displaced quencher strand can no longer anneal to fully synthesised duplex RNA leading to an increase in Cy3 fluorescent signal. ( E ) FRET-based strand displacement assay using the indicated concentrations of Sf nsp12-F/7H8.
438 C Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/pFastBac+His6+TEV+cloning+vector+with+BioBrick+PolyPromoter+LIC+Subcloning+(438-B)+(Plasmid+%2355219)/bio_rxiv__2024__07__18__603998-42-5-12
Average 93 stars, based on 1 article reviews
438 c vector - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
vector 48324  (Addgene inc)
90
Addgene inc vector 48324
( A ) Purified SARS-CoV-2 RdRp proteins expressed in baculovirus-infected insect cells ( Spodoptera frugiperda , Sf) analysed by SDS–PAGE and Coomassie staining. 7H8: nsp7-His 6 -nsp8, nsp12-HF: nsp12-His 6 <t>-3xFlag,</t> nsp12-F/7H8: nsp12-3xFlag/nsp7-His 6 -nsp8, nsp12-HF/7L8: nsp12-His 6 -3xFlag/nsp7-GGSGGS-nsp8, nsp12-HF/7/8: nsp12-His 6 -3xFlag/nsp7/nsp8. ( B ) Bacterially expressed and purified SARS-CoV-2 nsp7, nsp8 and nsp12 proteins analysed by SDS–PAGE and Coomassie staining. The proteins were expressed as 14His-SUMO fusion proteins in E. coli . 14His-SUMO was removed by a SUMO-specific protease during purification generating native N-termini. ( C ) Gel-based primer-extension assay to test RNA-dependent RNA synthesis using the RdRp preparation Sf nsp12-F/7H8. The substrate consists of a 10 nt RNA primer annealed to the 3′ end of a 35 nt RNA template. The 5′ end of the template strand is labelled with a Cy3 fluorophore. Reaction products were analysed by native PAGE and visualisation of Cy3 fluorescence. Formation of duplex RNA by RdRp was observed over time. Controls: a preformed Cy3-labelled dsRNA with the same size as the reaction product (dsRNA), the primed substrate (no protein). ( D ) Schematic diagram illustrating the FRET-based RdRp strand displacement assay. The RNA substrate is composed of a Cy3 fluorophore-containing template strand, an annealed primer and an annealed quencher strand with a 5′ flap. RdRp activity synthesises RNA by extending the primer strand and displaces the quencher strand. The displaced quencher strand can no longer anneal to fully synthesised duplex RNA leading to an increase in Cy3 fluorescent signal. ( E ) FRET-based strand displacement assay using the indicated concentrations of Sf nsp12-F/7H8.
Vector 48324, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/pET+His6+TEV+co-transformation+cloning+vector+(13S-B)+(Plasmid+%2348324)/pmc06411835-49-14-13
Average 90 stars, based on 1 article reviews
vector 48324 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
uc berkeley macrolab vector 2bt  (Addgene inc)
94
Addgene inc uc berkeley macrolab vector 2bt
( A ) Purified SARS-CoV-2 RdRp proteins expressed in baculovirus-infected insect cells ( Spodoptera frugiperda , Sf) analysed by SDS–PAGE and Coomassie staining. 7H8: nsp7-His 6 -nsp8, nsp12-HF: nsp12-His 6 <t>-3xFlag,</t> nsp12-F/7H8: nsp12-3xFlag/nsp7-His 6 -nsp8, nsp12-HF/7L8: nsp12-His 6 -3xFlag/nsp7-GGSGGS-nsp8, nsp12-HF/7/8: nsp12-His 6 -3xFlag/nsp7/nsp8. ( B ) Bacterially expressed and purified SARS-CoV-2 nsp7, nsp8 and nsp12 proteins analysed by SDS–PAGE and Coomassie staining. The proteins were expressed as 14His-SUMO fusion proteins in E. coli . 14His-SUMO was removed by a SUMO-specific protease during purification generating native N-termini. ( C ) Gel-based primer-extension assay to test RNA-dependent RNA synthesis using the RdRp preparation Sf nsp12-F/7H8. The substrate consists of a 10 nt RNA primer annealed to the 3′ end of a 35 nt RNA template. The 5′ end of the template strand is labelled with a Cy3 fluorophore. Reaction products were analysed by native PAGE and visualisation of Cy3 fluorescence. Formation of duplex RNA by RdRp was observed over time. Controls: a preformed Cy3-labelled dsRNA with the same size as the reaction product (dsRNA), the primed substrate (no protein). ( D ) Schematic diagram illustrating the FRET-based RdRp strand displacement assay. The RNA substrate is composed of a Cy3 fluorophore-containing template strand, an annealed primer and an annealed quencher strand with a 5′ flap. RdRp activity synthesises RNA by extending the primer strand and displaces the quencher strand. The displaced quencher strand can no longer anneal to fully synthesised duplex RNA leading to an increase in Cy3 fluorescent signal. ( E ) FRET-based strand displacement assay using the indicated concentrations of Sf nsp12-F/7H8.
Uc Berkeley Macrolab Vector 2bt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/pET+His6+TEV+LIC+cloning+vector+(2B-T)+(Plasmid+%2329666)/pmc09122588-32-32-37
Average 94 stars, based on 1 article reviews
uc berkeley macrolab vector 2bt - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
pfastbac 6xhis mbp  (Addgene inc)
93
Addgene inc pfastbac 6xhis mbp
( A ) Purified SARS-CoV-2 RdRp proteins expressed in baculovirus-infected insect cells ( Spodoptera frugiperda , Sf) analysed by SDS–PAGE and Coomassie staining. 7H8: nsp7-His 6 -nsp8, nsp12-HF: nsp12-His 6 <t>-3xFlag,</t> nsp12-F/7H8: nsp12-3xFlag/nsp7-His 6 -nsp8, nsp12-HF/7L8: nsp12-His 6 -3xFlag/nsp7-GGSGGS-nsp8, nsp12-HF/7/8: nsp12-His 6 -3xFlag/nsp7/nsp8. ( B ) Bacterially expressed and purified SARS-CoV-2 nsp7, nsp8 and nsp12 proteins analysed by SDS–PAGE and Coomassie staining. The proteins were expressed as 14His-SUMO fusion proteins in E. coli . 14His-SUMO was removed by a SUMO-specific protease during purification generating native N-termini. ( C ) Gel-based primer-extension assay to test RNA-dependent RNA synthesis using the RdRp preparation Sf nsp12-F/7H8. The substrate consists of a 10 nt RNA primer annealed to the 3′ end of a 35 nt RNA template. The 5′ end of the template strand is labelled with a Cy3 fluorophore. Reaction products were analysed by native PAGE and visualisation of Cy3 fluorescence. Formation of duplex RNA by RdRp was observed over time. Controls: a preformed Cy3-labelled dsRNA with the same size as the reaction product (dsRNA), the primed substrate (no protein). ( D ) Schematic diagram illustrating the FRET-based RdRp strand displacement assay. The RNA substrate is composed of a Cy3 fluorophore-containing template strand, an annealed primer and an annealed quencher strand with a 5′ flap. RdRp activity synthesises RNA by extending the primer strand and displaces the quencher strand. The displaced quencher strand can no longer anneal to fully synthesised duplex RNA leading to an increase in Cy3 fluorescent signal. ( E ) FRET-based strand displacement assay using the indicated concentrations of Sf nsp12-F/7H8.
Pfastbac 6xhis Mbp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his6+sumo+tev+fusion+vector/pFastBac+His6+MBP+N10+TEV+LIC+cloning+vector+(4C)+(Plasmid+%2330116)/pmc09097542-73-0-2
Average 93 stars, based on 1 article reviews
pfastbac 6xhis mbp - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


( A ) Purified SARS-CoV-2 RdRp proteins expressed in baculovirus-infected insect cells ( Spodoptera frugiperda , Sf) analysed by SDS–PAGE and Coomassie staining. 7H8: nsp7-His 6 -nsp8, nsp12-HF: nsp12-His 6 -3xFlag, nsp12-F/7H8: nsp12-3xFlag/nsp7-His 6 -nsp8, nsp12-HF/7L8: nsp12-His 6 -3xFlag/nsp7-GGSGGS-nsp8, nsp12-HF/7/8: nsp12-His 6 -3xFlag/nsp7/nsp8. ( B ) Bacterially expressed and purified SARS-CoV-2 nsp7, nsp8 and nsp12 proteins analysed by SDS–PAGE and Coomassie staining. The proteins were expressed as 14His-SUMO fusion proteins in E. coli . 14His-SUMO was removed by a SUMO-specific protease during purification generating native N-termini. ( C ) Gel-based primer-extension assay to test RNA-dependent RNA synthesis using the RdRp preparation Sf nsp12-F/7H8. The substrate consists of a 10 nt RNA primer annealed to the 3′ end of a 35 nt RNA template. The 5′ end of the template strand is labelled with a Cy3 fluorophore. Reaction products were analysed by native PAGE and visualisation of Cy3 fluorescence. Formation of duplex RNA by RdRp was observed over time. Controls: a preformed Cy3-labelled dsRNA with the same size as the reaction product (dsRNA), the primed substrate (no protein). ( D ) Schematic diagram illustrating the FRET-based RdRp strand displacement assay. The RNA substrate is composed of a Cy3 fluorophore-containing template strand, an annealed primer and an annealed quencher strand with a 5′ flap. RdRp activity synthesises RNA by extending the primer strand and displaces the quencher strand. The displaced quencher strand can no longer anneal to fully synthesised duplex RNA leading to an increase in Cy3 fluorescent signal. ( E ) FRET-based strand displacement assay using the indicated concentrations of Sf nsp12-F/7H8.

Journal: Biochemical Journal

Article Title: Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp12/7/8 RNA-dependent RNA polymerase

doi: 10.1042/BCJ20210200

Figure Lengend Snippet: ( A ) Purified SARS-CoV-2 RdRp proteins expressed in baculovirus-infected insect cells ( Spodoptera frugiperda , Sf) analysed by SDS–PAGE and Coomassie staining. 7H8: nsp7-His 6 -nsp8, nsp12-HF: nsp12-His 6 -3xFlag, nsp12-F/7H8: nsp12-3xFlag/nsp7-His 6 -nsp8, nsp12-HF/7L8: nsp12-His 6 -3xFlag/nsp7-GGSGGS-nsp8, nsp12-HF/7/8: nsp12-His 6 -3xFlag/nsp7/nsp8. ( B ) Bacterially expressed and purified SARS-CoV-2 nsp7, nsp8 and nsp12 proteins analysed by SDS–PAGE and Coomassie staining. The proteins were expressed as 14His-SUMO fusion proteins in E. coli . 14His-SUMO was removed by a SUMO-specific protease during purification generating native N-termini. ( C ) Gel-based primer-extension assay to test RNA-dependent RNA synthesis using the RdRp preparation Sf nsp12-F/7H8. The substrate consists of a 10 nt RNA primer annealed to the 3′ end of a 35 nt RNA template. The 5′ end of the template strand is labelled with a Cy3 fluorophore. Reaction products were analysed by native PAGE and visualisation of Cy3 fluorescence. Formation of duplex RNA by RdRp was observed over time. Controls: a preformed Cy3-labelled dsRNA with the same size as the reaction product (dsRNA), the primed substrate (no protein). ( D ) Schematic diagram illustrating the FRET-based RdRp strand displacement assay. The RNA substrate is composed of a Cy3 fluorophore-containing template strand, an annealed primer and an annealed quencher strand with a 5′ flap. RdRp activity synthesises RNA by extending the primer strand and displaces the quencher strand. The displaced quencher strand can no longer anneal to fully synthesised duplex RNA leading to an increase in Cy3 fluorescent signal. ( E ) FRET-based strand displacement assay using the indicated concentrations of Sf nsp12-F/7H8.

Article Snippet: The vectors pBIG1a_nsp12-His 6 -3xFlag, pBIG1b_nsp7-Linker-nsp8, and empty pBIG1c were used to generate pBIG2abc_nsp12-His 6 -3xFlag/nsp7-Linker-nsp8 (nsp12-HF/7L8, Addgene ID: 169184).

Techniques: Purification, Infection, SDS Page, Staining, Primer Extension Assay, Clear Native PAGE, Fluorescence, Activity Assay